Abstract
A monkey cDNA (pDTS) encoding a diphtheria toxin (DT) sensitivity determinant was isolated by expression cloning in mouse L-M cells. Mouse cells are naturally resistant to DT, because they lack functional cell surface receptors for the toxin. Unlike wild-type L-M cells, pDTS-transfected mouse cells are extremely toxin sensitive and specifically bind radioiodinated DT. Intoxication of the transfected cells requires receptor-mediated endocytosis of the bound toxin. The cDNA is predicted to encode an integral membrane protein that is identical to the precursor of a heparin-binding EGF-like growth factor. The DT sensitivity protein is thus a growth factor precursor that DT exploits as a receptor.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Animals
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Base Sequence
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Chlorocebus aethiops
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Cloning, Molecular
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Cross-Linking Reagents
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DNA / genetics
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Diphtheria Toxin / metabolism*
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Gene Expression
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Growth Substances / genetics*
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Heparin / metabolism
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Heparin-binding EGF-like Growth Factor
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Hydrogen-Ion Concentration
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Intercellular Signaling Peptides and Proteins
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Membrane Proteins / genetics*
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Mice
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Molecular Sequence Data
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RNA, Messenger / genetics
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Receptors, Cell Surface*
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Receptors, Cholinergic / genetics*
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Transfection
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Trypsin / pharmacology
Substances
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Cross-Linking Reagents
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Diphtheria Toxin
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Growth Substances
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Hbegf protein, mouse
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Heparin-binding EGF-like Growth Factor
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Intercellular Signaling Peptides and Proteins
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Membrane Proteins
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RNA, Messenger
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Receptors, Cell Surface
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Receptors, Cholinergic
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Heparin
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DNA
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Trypsin
Associated data
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GENBANK/M60278
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GENBANK/M84120
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GENBANK/M84163
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GENBANK/M84182
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GENBANK/M93012
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GENBANK/S72766
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GENBANK/S72767
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GENBANK/S72768
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GENBANK/S72769
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GENBANK/S72771
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GENBANK/X63098