Medline ® Abstract for Reference 40
of 'Therapeutic endoscopic ultrasound'
EUS-guided photodynamic therapy with verteporfin for ablation of normal pancreatic tissue: a pilot study in a porcine model (with video).
Yusuf TE, Matthes K, Brugge WR
Gastrointest Endosc. 2008 May;67(6):957-61. Epub 2008 Feb 21.
BACKGROUND: EUS-guided photodynamic therapy (PDT) with the photosensitizing agent porfimer sodium has been shown to be effective in ablation of pancreatic tissue.
OBJECTIVE: The objective of this study was to determine the effectiveness and safety of EUS PDT with verteporfin, a photosensitizer associated with less photosensitivity.
DESIGN: Prospective investigation of 6 swine that received an IV injection of 6 mg/m2 of verteporfin before EUS.
SETTING: Hospital animal laboratory.
INTERVENTIONS: The tail of the pancreas was located with EUS and was used to guide the placement of a light catheter. The pancreatic tail was exposed to 10, 15, or 20 minutes of laser light (689 nm).
MAIN OUTCOME MEASUREMENTS: Follow-up abdominal CT, liver and renal function tests, and serum pancreatic enzymes levels were performed. Histology of the pancreas was obtained 7 days after the procedure on necropsy.
RESULTS: Localized tissue necrosis within the pancreatic tail (range 6.6-30.5 mm in diameter) was seen in all animals. The diameter of the necrotic tissue was directly related to the dose of light. No post-procedural complications were observed.
LIMITATIONS: Normal animal model (lack of malignant tissue).
CONCLUSIONS: EUS-guided PDT of porcine pancreas with verteporfin achieved localized pancreatic tissue ablation in a dose-related fashion.
GI Unit, Massachusetts General Hospital, Boston, Massachusetts 02114, USA.