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Red blood cell compatibility testing (crossmatching)

INTRODUCTION

Compatibility testing is performed by the transfusion service in an effort to detect any serologic incompatibilities that might result in decreased survival of donor red cells in the transfused patient. The following tests are required [1,2]:

ABO confirmation and Rh determination of the recipient's red cells

ABO confirmation and Rh confirmation of the donor red cells if labeled as Rh negative

Antibody screen of the recipient's serum to detect clinically significant antibodies (ie, the indirect Coombs test)

Crossmatch of the recipient's serum with the donor red cells by a method that will detect ABO incompatibility and clinically significant antibodies

                   

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Literature review current through: Oct 2014. | This topic last updated: Aug 26, 2013.
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References
Top
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  2. Yazer MH. The blood bank "black box" debunked: pretransfusion testing explained. CMAJ 2006; 174:29.
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  12. Shulman IA, Petz LD. Red cell compatibility testing: Clinical significance and laboratory methods. In: Clinical practice of transfusion medicine, Petz LD, Swisher SN, Kleinman S, et al (Eds), Churchill and Livingstone, San Francisco 1996.
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  14. Ludvigsen CW Jr, Swanson JL, Thompson TR, McCullough J. The failure of neonates to form red blood cell alloantibodies in response to multiple transfusions. Am J Clin Pathol 1987; 87:250.
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