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Medline ® Abstract for Reference 11

of 'Rapid drug desensitization for immediate hypersensitivity reactions'

11
TI
Desensitization of human basophils with suboptimal concentrations of agonist. Evidence for reversible and irreversible desensitization.
AU
Pruzansky JJ, Patterson R
SO
Immunology. 1988;65(3):443.
 
Leucocytes from allergic donors were preincubated with suboptimal concentrations of ragweed or anti-IgE and then challenged with increasing concentrations of the homologous or heterologous agonist. The initial incubation resulted in desensitization, as judged by a reduced reactivity relative to controls preincubated without agonist but challenged similarly. Both homologous and heterologous desensitization were observed and were dose dependent. Evidence was obtained for both a reversible and irreversible component of desensitization, which was also agonist-concentration related. Reversibility occurred to a similar degree either by incubation of suboptimally desensitized cells with optimal concentrations of agonist or by removal of IgE and resensitization. This could implicate IgE-agonist aggregation on the basophil surface as a mechanism of desensitization. Histamine release from desensitized cells was highly correlated with degranulation, suggesting that individual cells were desensitized in an all-or-none manner.
AD
Department of Medicine, Northwestern University Medical School, Chicago, Illinois 60611.
PMID