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Medline ® Abstract for Reference 71

of 'Principles of cancer immunotherapy'

4-1BB protects dendritic cells from prostate cancer-induced apoptosis.
Youlin K, Jianwei Z, Xin G, Li Z, Xiaodong W, Xiuheng L, Hengchen Z, Zhiyuan C
Pathol Oncol Res. 2013 Apr;19(2):177-81. Epub 2012 Oct 16.
It has been shown that human prostate cancer (PCa) cells induced apoptotic death of the most potent antigen-presenting cells, dendritic cells (DCs), which are responsible for the induction of specific antitumor immune responses. Here, we investigated the function of 4-1BB on protecting DCs from prostate cancer-induced apoptosis with an agonistic mAb to 4-1BB. RM-1 cells and DCs were co-incubated for 48 h and DC apoptosis was assessed by Annexin Vassay. TNF-αand IL-12 production were assessed by enzyme-linked immunosorbent assay (ELISA) and Bcl-2 and Bcl-xL on DCs were analyzed by Western blot. We have shown that co-incubation of RM-1 cells with DCs is accompanied by an increased level of DCs apoptosis. Triggering 4-1BB on DCs resulted in increased resistance of DCs to RM-1 cells-induced apoptosis, which was owing to the up-regulated expression of Bcl-2 and Bcl-xL, and increased secretion of TNF-αand IL-12. These results demonstrate that triggering 4-1BB on DCs could increased resistance of DCs to PCa-induced apoptosis.
Department of Urology, The First Affiliated Hospital, Chongqing Medical University, Chongqing, 400016, China.