Anees B Chagpar, MD, MSc, MA, MPH, MBA, FACS, FRCS(C)
Daniel F Hayes, MD
Lori J Pierce, MD
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INTRODUCTION — The status of the axillary lymph nodes is one of the most important prognostic factors in patients with breast cancer. Histologic examination of lymph nodes is the most accurate method for assessing lymph node metastasis. Axillary dissection has traditionally been used in breast surgery to obtain lymph nodes. Sentinel lymph node biopsy has replaced axillary lymph node dissection in many patients with early breast cancer. (See "Management of the regional lymph nodes in breast cancer" and "Prognostic and predictive factors in metastatic breast cancer", section on 'Prognostic versus predictive factors'.)
Indications for and outcomes of sentinel lymph node biopsy will be reviewed here. The technique of sentinel lymph node mapping in patients with breast cancer is discussed separately. (See "Sentinel lymph node biopsy in breast cancer: Techniques".)
PREOPERATIVE AXILLA EVALUATION — Preoperative evaluation of axillary lymph nodes separates patients into two categories: patients with clinically positive nodes undergo axillary lymph node dissection (ALND), and patients with clinically negative nodes undergo sentinel lymph node biopsy (SLNB).
It is essential that patients receive adequate evaluation of the axilla prior to surgery. Patients who have been suboptimally examined for clinically involved nodes before surgery are at a greater risk for recurrence. The applicability of SLNB is based on trials that only included clinically node-negative patients. ALND remains the standard of care for patients with clinically positive nodes.
Palpable node — Patients with a palpably enlarged, firm mobile lymph node who elect not to undergo preoperative biopsy should undergo ALND with their primary breast procedure. Those who do elect to have preoperative biopsy may undergo core needle biopsy or fine needle aspiration of the palpable lymph nodes for pathologic analysis [1-7]. Patients who have biopsy-confirmed nodal disease should undergo ALND for axillary clearance.
SLNB with simultaneous removal of the palpable node(s) is an acceptable option for staging these patients with palpable nodes in whom the preoperative biopsy does not confirm metastatic disease. If subsequent pathologic evaluation of the palpable node(s) demonstrates metastatic disease, the patient should be considered clinically node-positive when deciding how to further manage the remaining axillary nodes.
Abnormal node on imaging — When clinical examination alone is suboptimal (eg, in obese patients), patients may benefit from axillary imaging. Axillary ultrasound is an effective screening method for detecting nonpalpable nodal metastasis in patients with invasive breast cancer.
In one study of 653 consecutive patients, ultrasound-guided fine needle aspiration diagnosed axillary disease in 23 percent of patients, thereby negating a second operation in 150 . However, the efficacy of ultrasound-guided biopsy can vary between centers because the accuracy of ultrasound examination is operator dependent . (See "Diagnostic evaluation of women with suspected breast cancer" and "Breast biopsy".)
Patients with abnormal axillary lymph nodes by imaging have a higher risk of having multiple metastatic lymph nodes . Such patients should undergo core biopsy or fine needle aspiration of the suspicious node and, if positive, an ALND for axillary clearance .
Indications — We recommend the use of sentinel lymph node biopsy (SLNB) to assess axillary lymph nodes in patients with early breast cancer (T1 or T2) who do not have clinically positive nodes and in patients with ductal carcinoma in situ (DCIS) when mastectomy is performed. These recommendations are consistent with those made by the American Society of Clinical Oncology in 2014 .
Early breast cancer with clinically negative node — SLNB should be performed in women with clinically node-negative early breast cancer . For these patients, SLNB is a method of staging the axilla with less morbidity than axillary lymph node dissection (ALND).
DCIS with planned mastectomy or suspicious features — Most women with DCIS do not require assessment of the axillary nodes, because DCIS is not invasive cancer and does not metastasize. However, two groups of women undergoing breast surgery for DCIS may benefit from SLNB.
DCIS with planned mastectomy — SLNB should be performed in all patient with DCIS undergoing total mastectomy. The technique of SLNB involves injecting radiolabeled tracer into the breast parenchyma near the area of DCIS or, alternatively, in a periareolar location. After a total mastectomy, the lymphatic drainage pattern will be permanently altered, making it impossible to accurately perform SLNB at a later date if invasive cancer is found unexpectedly in the mastectomy specimen .
DCIS with suspicious features — Some also recommend SLNB to patients undergoing breast-conserving surgery for DCIS clinically suspected of harboring invasive cancer, including DCIS larger than 5 cm and DCIS with a palpable mass. Offering SLNB with breast-conserving surgery to DCIS patients who have a high clinical or pathological suspicion of synchronous invasive cancer may spare them the inconvenience of a second operation.
This practice, however, is controversial . Invasive carcinoma has only been reported in 10 to 20 percent of excision specimens following a biopsy diagnosis of DCIS . Thus, most women will not have any invasive disease on final pathology. For them, omitting SLNB at the time of their breast-conserving surgery eliminates any perioperative morbidity associated with SLNB [14-16]. If invasive breast cancer is identified after a breast-conserving surgery is performed for DCIS, SLNB can be performed as a second procedure to stage the axilla. Disease stage and subsequent axillary management may change for such patients [15,16]. (See "Breast ductal carcinoma in situ: Epidemiology, clinical manifestations, and diagnosis" and "Ductal carcinoma in situ: Treatment and prognosis" and "Microinvasive breast carcinoma" and "Tumor, Node, Metastasis (TNM) staging classification for breast cancer".)
Contraindications — Clinically positive nodes and inflammatory breast cancer (T4d) are absolute contraindications for SLNB; both should be treated with ALND . Locally advanced breast cancer is a relative contraindication for SLNB. Certain large tumors (eg, T3) may be amenable to SLNB, while others with skin or chest wall involvement (eg, T4a-c) should be treated with ALND (table 1). Those who undergo neoadjuvant therapy for locally advanced disease should be re-evaluated for the suitability of SLNB prior to surgery. Additionally, SLNB should be omitted in patients whose axillary status does not guide adjuvant therapy.
Clinically positive nodes — We recommend that patients who have clinically positive nodes that have been pathologically proven to contain metastatic cancer should undergo axillary lymph node dissection (ALND) for axillary clearance, rather than SLNB.
Locally advanced and inflammatory breast cancer — The 2014 American Society of Clinical Oncology and the 2010 International Expert Panel guidelines on SLNB recommend against the use of SLNB in patients with locally advanced or inflammatory breast cancer [10,14,17]. Axillary lymph node dissection (ALND) is recommended for this group of patients to maximize locoregional control .
Since larger tumors have a higher likelihood of metastasizing to axillary nodes, most trials have restricted SLNB use to T1 or T2 breast cancers (<5 cm) (table 1). However, some studies have shown that SLNB can be accurate in patients with T3 tumors and clinically negative axilla [18,19]. Thus, many clinicians, including the authors of this topic, do not recognize tumor size of larger than 5 cm (T3) as an absolute contraindication to SLNB, as long as the axilla is clinically negative.
Patients with locally advanced tumors with skin and/or chest wall involvement (T4a-c) or inflammatory breast cancer (T4d) are not candidates for SLNB [20,21]. The false negative rate is high in such patients, presumably because of partially obstructed and/or functionally abnormal subdermal lymphatics.
Patients whose axillary status does not guide adjuvant treatment — We do not consider advanced age an absolute contraindication to SLNB in women with breast cancer, as age has never been shown to influence the accuracy, safety, and value of SLNB. However, SLNB should be omitted if the nodal information gained through the procedure will not affect adjuvant treatment decisions.
As an example, women over the age of 70 years with T1 cancers that are estrogen receptor-positive and clinically node-negative have excellent overall survival rates and distant disease-free survival rates when treated with lumpectomy and tamoxifen, with or without breast irradiation .
We suggest that the decision to omit SLNB in older women be made in a multidisciplinary setting with input from the treating medical and radiation oncologists to ensure all providers are in agreement as to whether or not axillary staging information is important for adjuvant therapy decisions. This is particularly true for patients who are between 65 and 70 years old. Many surgeons would perform SLNB and completion ALND for healthy women at that age or sometimes older, depending upon their overall state of health, taking into consideration the patient's preference as well. The management of early breast cancer in older women is discussed in detail elsewhere. (See "General principles on the treatment of early-stage and locally advanced breast cancer in older women", section on 'Management of the axilla'.)
Special circumstances — Although SLNB has been performed in the following special circumstances, its use remains controversial and not universally accepted .
Neoadjuvant chemotherapy — Neoadjuvant systemic therapy is the accepted approach for women with locally advanced breast cancer, women with inflammatory breast cancer, and women who have operable breast cancer but desire breast-conserving surgery. It is controversial whether SLNB or ALND should be performed and whether it should be performed prior to or following the completion of neoadjuvant chemotherapy in such patients. (See "General principles of neoadjuvant therapy for breast cancer", section on 'Node evaluation'.)
Multicentric disease — Multicentric disease is not a contraindication to SLNB . Studies of breast lymphatic drainage indicate that all quadrants of the breast drain into the same lymph node(s) [23,24]. Thus, subareolar and intradermal (rather than peritumoral) injection of radiolabeled colloid or blue dye make SLNB feasible for patients with multicentric disease [23,25-27]. (See "Sentinel lymph node biopsy in breast cancer: Techniques".)
Successful SLNB for multicentric disease has been demonstrated by several studies [26,28]. In one study of 142 women with multicentric breast cancer, SLNB was successful in 91 percent of patients, with a false negative rate of 4 percent . However, more patients undergo completion ALND after SLNB in multicentric compared with unicentric disease. The likelihood of finding additional disease at the time of completion ALND is also higher with multicentric disease.
Previous breast and axillary procedures for benign conditions — Two studies have demonstrated feasibility and accuracy of SLNB in patients who had prior excisional breast biopsy [29,30]. However, it is unclear whether women who have undergone cosmetic breast surgeries, such as reduction mammoplasty or breast augmentation, can undergo SLNB. Patients who have had extensive breast or axillary surgery may have disruption or alteration to the normal pattern of lymphatic drainage, which may increase the false negative rate of SLNB. Thus, in these patients, we perform preoperative lymphoscintigraphy prior to SLNB. (See "Sentinel lymph node biopsy in breast cancer: Techniques".)
Recurrent breast cancer and previous axillary procedures — SLNB after previous axillary surgery has not been widely studied. However, there are accumulating reports of successful second SLNB in patients with local recurrences of breast cancer following a previous SLNB or ALND [31-34].
In women who were previously treated with breast-conserving therapy and have in-breast recurrence or a new ipsilateral breast cancer, we perform a lymphoscintigraphy to identify the sentinel node(s) before attempting a repeat SLNB. Technical failure rates and false negative rates of a SLNB in this setting are related to the extent of previous axillary surgery and are in general higher compared with an initial SLNB. (See "Management of locoregional recurrence of breast cancer after breast conserving therapy".)
Although the success rate of identifying a sentinel node is lower with prior axillary surgery, SLNB is as accurate as in patients without prior axillary surgery when a sentinel node can be identified [35,36]. In a meta-analysis of over 1000 patients undergoing SLNB for recurrent breast cancer, sentinel nodes were identified intraoperatively in 60 percent (95% CI 57 to 63 percent) of patients . Aberrant lymphatic drainage pattern was detected in 26 percent (95% CI 23 to 28 percent), less frequently after SLNB than after ALND (OR 0.27, 95% CI 0.19-0.38). The metastatic sentinel node rate was 10 percent (95% CI 8.6 to 12.3 percent).
Male breast cancer — The vast majority of published studies of SLNB for breast cancer were done in women. Data are limited in men with breast cancer because they are uncommon. A retrospective study of 30 men with breast cancer reported a 100 percent SLNB identification rate and a false negative rate of 0 percent . Prospective studies establishing the sensitivity and specificity of SLNB in male breast cancer patients have not been carried out. However, we feel it is acceptable to proceed with SLNB in men and that the principles guiding SLNB in women appear to apply to men.
Male breast cancer is discussed in detail elsewhere. (See "Breast cancer in men", section on 'Management of the regional nodes'.)
Pregnancy — SLNB is best avoided in women who are pregnant because of potential teratogenic effects on the developing fetus from isosulfan blue dye and a lack of safety studies on other tracer agents [10,39]. When axillary surgery is necessary during pregnancy, limited data suggest that SLNB can be carried out, but with radioactive colloid alone, which is not teratogenic at the dose administered for SLNB.
In one retrospective study, 25 pregnant women with breast cancer were injected with methylene blue (7), radioactive colloid (16), or another type of tracer (2) during SLNB procedures . There were 25 livebirth infants, 24 of whom were deemed healthy at delivery; one infant had a cleft lip not attributed to the injections. At 2.5 years, no adverse outcomes were attributed to the injection. Other studies also demonstrated that the dose of radiation from radioactive colloid to the fetus is minimal and that SLNB can be safely performed during pregnancy using radiolabeled tracers .
Although some consider the use of methylene blue or radioactive colloid to be safe to the fetus in pregnant women with breast cancer, isosulfan blue dye (Lymphazurin) is systemically absorbed after subcutaneous injection and therefore should not be used during pregnancy [42,43]. (See "Gestational breast cancer: Treatment", section on 'Management of the axilla'.)
VALIDATION AND PATHOLOGY — Sentinel lymph node biopsy (SLNB) utilizes the lymphatic mapping technique to locate and remove one or more (on average, three) axillary lymph nodes in patients with breast cancer . The sentinel lymph nodes are then submitted for pathologic examination, providing vital information about the axillary nodal status of the patient and guiding further treatment. (See "Management of the regional lymph nodes in breast cancer".)
The SLNB technique is discussed elsewhere. (See "Sentinel lymph node biopsy in breast cancer: Techniques".)
Development and validation of SLNB technique — The SLNB technique has been developed and validated over the past three decades. It has been demonstrated to be feasible, accurate, and less morbid than axillary lymph node dissection (ALND). It has been adopted by medical centers worldwide as the standard initial approach for patients with early-stage breast cancer.
Despite variability in selection criteria and technique, a sentinel lymph node is consistently identified in approximately 96 percent of patients and predicts the status of the remaining axillary lymph nodes in ≥95 percent of patients in most series [45,46].
A systematic review of 69 trials of SLNB, including 8059 patients, showed that sentinel lymph nodes could be identified in 95 percent of patients with a false negative rate of 7.3 percent (range 0 to 29 percent) [14,47]. Using a combination of isosulfan blue dye and radioactive colloid resulted in a significantly higher success rate and lower false negative rate in sentinel node mapping compared with using isosulfan blue dye alone.
The false negative rate of SLNB was originally reported as being 5 to 10 percent (sensitivity 90 to 95 percent), but lower rates were attained by experienced surgeons [45,48]. Despite initial concerns that this false negative rate would translate into increased axillary recurrence, subsequent trials found that recurrence rates with SLNB were comparable to those with ALND [44,49].
In the National Surgical Adjuvant Breast and Bowel Project (NSABP) B-32 trial, for example, axillary recurrence after SLNB was 0.4 percent with ALND versus 0.7 percent without ALND . Similar findings were demonstrated in the Veronesi study (0 percent with ALND versus 0.8 percent without ALND) . The low axillary recurrence rates in these trials, even without ALND, were presumably due to the effects of adjuvant therapy (chemotherapy or radiotherapy) that may treat residual axillary disease burden. In patients who did not receive adjuvant therapy or ALND, the axillary recurrence rate was as high as 20 percent, as reported for the NSABP-04 trial .
SLNB is less morbid for patients than ALND. Several studies have shown that the risk of arm morbidity, particularly lymphedema, sensory loss, and shoulder abduction deficits, is significantly less for SLNB than ALND (table 2) [51-57]. As an example, in one trial, the risk of lymphedema after 12 months was reported as 2 percent after SLNB alone as compared with 13 percent after SLNB with ALND . In addition, SLNB identifies patients without axillary node involvement, thereby obviating the need for more extensive surgery for those patients .
The SLNB technique has been adopted by virtually all medical centers in the world as a standard means of axillary nodal assessment [58-60]. It is endorsed by multiple guidelines as an alternative to ALND for the diagnosis of axillary metastases in patients with clinically node-negative early breast cancer [10,11,14,61,62].
Pathologic analysis of nodal metastases — Sentinel node metastases are subgrouped into isolated tumor cell clusters, micrometastases, and macrometastases based upon the size of the largest contiguous tumor deposit present in the sentinel node, as determined by routine histologic examination of slides stained by hematoxylin and eosin (table 1).
Immunohistochemistry staining with cytokeratin is not routinely indicated in the pathologic examination of sentinel lymph nodes for breast cancer. Immunohistochemistry stains can be helpful in establishing the size and extent of metastatic disease and in confirming invasive, metastatic lobular carcinoma, a cancer subtype with morphology that can be difficult to discern by hematoxylin and eosin stain alone [63,64]. The use of immunohistochemistry staining, however, is reserved for cases in which metastatic cancer of any subtype, including lobular, is suspected but cannot be confirmed based on hematoxylin and eosin stain alone.
Isolated tumor cell clusters — Isolated tumor cell clusters are defined as small clusters of tumor cells not greater than 0.2 mm or nonconfluent or nearly confluent clusters of cells not exceeding 200 cells in a single histologic lymph node cross section . According to the Tumor, Node, Metastasis (TNM) staging system, isolated tumor cell clusters are designated as pN0(i+). Prognostically, patients with isolated tumor cell clusters appear to do as well as patients without any pathologic node involvement. Of note, the finding of isolated tumor cells or clusters in lymphatic channels as a result of iatrogenic displacement from core biopsy procedures has been observed but does not significantly change prognosis [66-69]. (See "Tumor, Node, Metastasis (TNM) staging classification for breast cancer".)
Micrometastases — Micrometastatic nodal involvement is defined as a metastatic deposit >0.2 mm but <2.0 mm. If present, it is designated as pN1mi in the American Joint Committee on Cancer (AJCC) staging system. Patients with pN1mi breast cancer have a slightly worse prognosis compared with those with node-negative breast cancer, but micrometastases do not predict recurrence . (See "Tumor, Node, Metastasis (TNM) staging classification for breast cancer" and "Prognostic and predictive factors in early, nonmetastatic breast cancer", section on 'Nodal involvement'.)
Macrometastases — Macrometastatic involvement of the axillary nodes (classically designated as "node-positive") is defined by any tumor cell deposit >2.0 mm. The presence of macrometastases within the axillary nodes is a well-established independent prognostic factor, with a worse prognosis associated with greater nodal involvement. (See "Prognostic and predictive factors in early, nonmetastatic breast cancer", section on 'Nodal involvement'.)
Extranodal extension — Extranodal (extracapsular) extension is defined as invasive tumor cells or clusters that are present outside of the lymph node capsule and parenchyma. In cases of extranodal extension, there is generally tumor present in both a subcapsular location and the adjacent extranodal fat. However, a fibrous reaction (desmoplasia) to the tumor cells may obscure tumor invasion of the extranodal fat.
Focal or minimal extracapsular extension of metastatic tumor is less significant than extensive or more obvious extranodal extension . Isolated tumor cells or clusters within perinodal or axillary lymphatic channels are not considered extranodal extension. Instead, they should be measured and classified as isolated tumor cell clusters (ITCs) or micrometastases. (See 'Isolated tumor cell clusters' above and 'Micrometastases' above.)
Occult metastatic disease — Occult micrometastases refer to nodal metastases that are not seen on initial hematoxylin and eosin examination but are detected subsequently by additional levels or by immunohistochemical staining or reverse transcriptase polymerase chain reaction. In one study, more intensive pathologic evaluation of the nodes by deeper sectioning and immunohistochemical staining increased the yield of occult metastases and led to an overall case conversion rate of 10.3 percent in patients who had an initial negative SLNB .
Occult micrometastases have no significance in terms of surgical management and patient outcomes [70,73]. Thus, routine immunohistochemistry staining or reverse transcriptase polymerase chain reaction is not recommended for the evaluation of sentinel lymph nodes in guidelines published by the American Society of Clinical Oncology, the National Comprehensive Cancer Network, and others [11,14,17]. Histologically negative nodes that are immunohistochemistry staining or reverse transcriptase polymerase chain reaction-positive are still classified as pN0 disease in the TNM staging system for breast cancer. (See "Tumor, Node, Metastasis (TNM) staging classification for breast cancer".)
MANAGEMENT AFTER SENTINEL LYMPH NODE BIOPSY — Sentinel lymph node biopsy (SLNB) has replaced axillary lymph node dissection (ALND) as the initial assessment of axillary nodes in patients with early breast cancer. Based upon pathologic results obtained by SLNB, we recommend no further axillary treatment for patients who meet all of the following criteria ("Z-0011-eligible" criteria) (see 'One or two sentinel node metastases' below):
●Clinically negative nodes based on an adequate clinical node evaluation, including imaging when necessary, such as in obese patients.
●A T1 or T2 (≤5 cm) primary breast cancer (table 1).
●Fewer than three metastatic sentinel lymph nodes on SLNB.
●Patients undergoing breast conserving surgery followed by whole-breast irradiation.
A completion ALND is required for patients who have:
●Three or more metastatic sentinel lymph nodes on SLNB.
●One or two metastatic sentinel lymph nodes on SLNB but who do not desire whole-breast irradiation.
These recommendations are consistent with the 2014 American Society of Clinical Oncology (ASCO) guidelines .
No sentinel node metastasis — We do not perform ALND in patients with early breast cancer who do not have nodal metastasis on SLNB, including those with isolated tumor cells present in the sentinel node(s).
Seven randomized trials compared patients with early breast cancer who underwent SLNB first followed by ALND only if SLNB was positive with those who underwent ALND (table 2). Compared with ALND, SLNB first followed by selective ALND in patients with positive sentinel lymph nodes resulted in similar overall survival (OS), disease-free survival (DFS), and disease recurrence rates but fewer adverse events and better quality of life. This conclusion is best illustrated by the results from the National Surgical Adjuvant Breast and Bowel Project (NSABP) B-32 trial, which included the largest number of patients of all the trials.
The NSABP B-32 trial enrolled 5611 early-stage breast cancer patients with clinically negative nodes and compared SLNB followed by ALND with SLNB followed by ALND only if the sentinel lymph node was positive [74,75]. SLNB was successfully performed in 97 percent of patients, and the false negative rate was 9.8 percent. No significant differences were observed in regional control, OS, or DFS between the groups at a median follow-up period of almost eight years [76,77].
Occult metastasis — Occult metastasis refers to tumor cells in the lymph node that are not seen on initial hematoxylin and eosin examination but are detected subsequently by examining additional levels or by performing immunohistochemistry staining or reverse transcriptase polymerase chain reaction (see 'Occult metastatic disease' above).
In patients with occult metastasis found by SLNB, no completion ALND is needed, and no alteration in subsequent adjuvant therapy is recommended [70,73,78-80].
Clinical trials have examined the significance of occult metastases in sentinel nodes. Blinded analysis of occult metastases in clinical trials provides prognostic data on the significance of micrometastases and isolated tumor cell clusters. The NSABP B-32 trial demonstrated that the presence of occult metastases was associated with a 1.2 percent lower OS rate and a 2.8 percent lower DFS rate; patients with occult metastases did not have a higher incidence of regional or distant recurrences . The American College of Surgeons Oncology Group (ACOSOG) Z-0010 trial found a 0.6 percent lower overall survival rate in patients with occult metastases, but this was not statistically significant . The two trials used different protocols for detecting occult metastases, and although the B-32 trial had higher statistical power, both trials demonstrated similar outcomes related to occult metastases. In addition, the occult metastases detected were almost entirely isolated tumor cell clusters or micrometastases and only rarely macrometastases .
In a retrospective study, 93,070 patients with invasive breast adenocarcinoma and negative lymph nodes on routine pathology underwent immunohistochemistry staining to evaluate for occult nodal disease. The presence of micrometastases was associated with a worse overall survival rate (hazard ratio [HR] 1.40, 95% CI 1.28-1.53), while the presence of isolated tumor cells was not (HR 1.05, 95% CI 0.92-1.20) .
One or two sentinel node metastases — There is compelling evidence that ALND is not necessary in most women with early-stage breast cancer who have only one or two sentinel lymph node metastases and who will receive whole-breast irradiation as part of breast-conserving therapy. If, however, whole-breast irradiation is not planned, then ALND is indicated for such patients.
Historically, patients with any nodal metastasis underwent completion ALND . However, only approximately 40 percent of patients with a positive sentinel lymph node had residual disease in the axilla [14,45-48,74,82-86]; the rest derived no benefit from the addition of ALND. Thus, several predictive nomograms were developed to estimate the risk of additional positive nodes based on known clinical and pathologic data derived from SLNB [87-94]. Most of these nomograms included data such as the size and number of the sentinel lymph node (SLN) metastases, extranodal extension, the size and presence of lymphovascular invasion in the primary tumor, and the size of the metastatic disease in the SLN . However, retrospective analyses of patients with positive SLNs who underwent completion ALND found none of the popular monograms to be sufficiently reliable for clinical use [90,95].
Two randomized trials, the ACOSOG Z-0011 trial and the International Breast Cancer Study Group 23-01 (IBCSG 23-01) trial, demonstrated that many of these patients with one or two metastatic sentinel nodes can safely avoid a completion axillary node dissection.
●The ACOSOG Z-0011 trial was designed to address the need for completion ALND for patients with T1 or T2 breast cancer who were clinically node-negative and had fewer than three positive sentinel nodes (table 2) [51,96,97]. All patients were treated with radiation to the whole breast. Compared with SLNB plus completion ALND, SLNB alone resulted in similar five-year OS rates (91.9 percent SLNB alone group versus 92.5 percent), DFS rates (82.2 SLNB alone group versus 83.9 percent), and recurrence events (four SLNB alone group versus two). The ACOSOG Z-0011 trial has been criticized for premature study termination due to low accrual and low event rates, protocol noncompliance, and high percentage (20 percent) of patients lost to follow-up [49,52]. In addition, the ACOSOG Z-0011 trial did not account for the number of patients in each study group who had isolated tumor cell clusters, micrometastases, or macrometastases. The majority of the study population had T1 (almost 70 percent) hormone receptor-positive (85 percent) tumors, which may influence survival outcomes in ways that cannot be controlled for. Nevertheless, the ACOSOG Z-0011 trial has widely changed the practice of early breast cancer treatment. Its results have been incorporated into treatment guidelines such as the American Society of Clinical Oncology 2014 practice guidelines for SLNB .
In a subsequent study of the same study population, patients in the two arms showed no statistically significant difference in local recurrence-free survival after a median follow-up of 9.25 years . The cumulative incidence of ipsilateral axillary recurrences at 10 years was 0.5 percent (2 patients) in the ALND arm and 1.5 percent (5 patients) in the SLND-alone arm; the 10-year cumulative incidence of local regional recurrences was 6.2 percent with ALND and 5.3 percent with SLND alone.
●The International Breast Cancer Study Group (IBCSG) 23-01 trial randomly assigned patients with a primary tumor <5 cm in size and clinically nonpalpable axillary nodes to either completion ALND or no additional axillary surgery after a finding of positive sentinel lymph node(s) (table 2) . Only one or two sentinel nodes were positive in 85 percent of the patients. All patients had nodal metastases less than 2 mm in size, and 67 percent had micrometastases less than 1 mm. Ninety percent of patients underwent breast-conserving surgery; 10 percent underwent mastectomy. A similar proportion of patients in each group received adjuvant radiation, hormonal therapy, and chemotherapy. Approximately 90 percent of patients in both groups received whole-breast irradiation. At five years, there was no statistically significant difference in DFS rates (84 percent with ALND versus 88 percent without ALND) with OS rates (98 percent versus 97.6 percent) between the two groups. The IBCSG 23-01 trial was also closed prematurely due to low enrollment and low event rate.
Because of these two randomized trials and other studies that examined the role of ALND in managing limited axillary disease in women with early-stage clinically node-negative breast cancer, the American Society of Clinical Oncology changed its guidelines from recommending ALND for all patients with a positive sentinel lymph node (2005) to recommending against ALND for patients with fewer than three positive sentinel lymph nodes (2014). As a result, the eligibility criteria for one of the trials have become descriptive for this group of patients . These so-called "Z-0011-eligible" patients are women with early-stage clinically node-negative breast cancer metastatic to one to two sentinel lymph nodes who undergo breast-conserving surgery with whole-breast radiotherapy. It is now a widespread practice that women with similarly favorable breast cancer no longer receive completion ALND.
Sentinel node metastasis with extranodal extension — Although the ACOSOG Z-0011 trial established that completion ALND is unnecessary for patients who have only one or two positive sentinel nodes, patients who had extranodal extension were excluded from the trial. Thus, completion ALND is still considered the standard of care for patients with extranodal extension of sentinel node metastasis, regardless of the number.
The presence of extranodal extension is associated with increased axillary nodal disease burden. The reported incidence of nonsentinel lymph node metastases in patients with extranodal extension ranged from 58 to 84 percent [99-101]. In contrast, the ACOSOG Z-0011 trial, which excluded patients with extranodal extension, reported nonsentinel nodal metastases in 27 percent of patients. In two studies, the presence of extranodal extension, regardless of size, was associated with a greater risk of having pN2 disease (four or more positive nodes) [71,102] (table 1).
Some, but not all, studies also reported increased disease recurrence [99,103] and decreased survival [99,104-108] with extranodal extension. However, none of these studies was prospective. Thus, most experts recommend that all patients who are found to have extranodal extension in any number of sentinel nodes should be offered completion ALND because of the high axillary nodal disease burden associated with extranodal extension.
Other experts suggested that only extranodal extension that is >2 mm should be considered an indication for ALND when applying Z0011 criteria to patients with fewer than three metastatic sentinel lymph nodes . In one study, 331 patients with pT1-2, cN0 disease with <3 positive SLNs underwent completion ALND. Thirty-three percent of patients with extranodal extension >2 mm had ≥4 additional positive nodes at completion ALND, compared with only 9 percent of patients with extranodal extension ≤2 mm. However, given that patients with any extranodal extension were excluded from the Z0011 trial, we recommend that all such patients should under completion ALND until further data on survival and recurrence rate become available.
Sentinel node metastasis with large tumor — Patients with large tumors (eg, T3) but clinically negative axilla were excluded from the ACOSOG Z-0011 trial. Although SLNB can be performed in such patients, conclusions drawn from the Z-0011 trial cannot be applied to such patients. Thus, completion ALND is still offered as the standard of care for any patient who has a large (>5 cm or T3) tumor (table 1) with any positive sentinel nodes. (See 'Locally advanced and inflammatory breast cancer' above.)
Three or more sentinel node metastases — For patients with three or more pathologically involved sentinel nodes, we recommend a completion ALND for staging purposes and to maximize local control [11,14]. The timing of the procedure (ie, immediate [one operation] versus delayed [two separate operations]) does not seem to affect the total lymph node yield or the rate of long-term complications (particularly lymphedema).
This recommendation is consistent with the 2014 guidelines from the American Society of Clinical Oncology  and is also supported by data from a 2013 systematic review that included 17 studies with at least two years of follow-up .
The use of ALND in breast cancer treatment is discussed elsewhere. (See "Technique of axillary lymph node dissection" and "Management of the regional lymph nodes in breast cancer".)
Role of radiotherapy — All of the women in the ACOSOG Z-0011 trial received whole-breast radiation, as did 90 percent of the women in the IBCSG 23-01 trial. Given that part of the axilla is included in the treatment field with whole-breast radiation, women who received radiation in the two trials probably experienced a therapeutic effect from radiation sterilizing any residual tumor cells in the axilla . The same benefit would not be conferred by partial-breast radiation unless axillary radiation is used separately to control the axilla . Thus, we recommend omitting ALND in patients with one or two positive SLNs who plan to undergo whole-breast irradiation but not partial-breast irradiation.
Reported in 2014, the After Mapping of the Axilla: Radiotherapy or Surgery (AMAROS) trial was a multi-institutional trial conducted by the European Organization for the Research and Treatment of Cancer (EORTC) . The trial included 4806 patients with T1 or T2, primary, unifocal, invasive breast cancer without palpable axillary lymphadenopathy. Of the 1425 patients who were found to have positive sentinel node(s) by SLNB, 744 were randomly assigned to receive ALND and 681 to receive axillary radiotherapy. Ninety-five percent of patients in both groups had one or two positive SLNs. The five-year axillary recurrence rate was 0.43 percent (0 to 0.92) in the ALND group and 1.19 percent (0.31 to 2.08) in the axillary radiation group. There were no statistically significant differences in DFS and OS between treatment groups. Lymphedema was noted significantly more often after ALND than after axillary radiation at one year, three years, and five years. This trial was limited by a low number of events and a short follow-up period.
The AMAROS trial showed axillary radiation to be an acceptable alternative to ALND in patients who have positive sentinel node(s) but do not meet the Z0011 criteria. For those who meet the Z0011 criteria, however, axillary radiation is likely to add toxicity without conferring any additional benefit compared with whole-breast irradiation alone. Additional studies are needed before regional radiotherapy can be accepted as a standard approach for patients with axillary metastases.
NONAXILLARY LYMPH NODE BIOPSY — Sentinel lymph node biopsy (SLNB) techniques can identify nonaxillary metastases (eg, internal mammary nodes and intramammary nodes) in up to 43 percent of patients, depending upon the volume/type of colloid injected, the injection technique [56-58], and the primary tumor location/size . Whether or not identifying nonaxillary sentinel nodes is useful remains controversial, since the majority of the data regarding treatment decisions and outcomes come from evaluation of only axillary nodes.
Internal mammary nodes — Internal mammary nodes are only visualized in 20 percent of patients during SLNB and can be difficult to remove because of their location. Thus, in the absence of definitive data, dissection of the internal mammary nodes with SLNB should be considered investigational.
There is no consensus on the need for internal mammary nodal dissection in women with detection of an internal mammary node by lymphoscintigraphy [14,60,61]. Several trials failed to demonstrate a survival benefit with surgical dissection of internal mammary nodes [110,111], and therefore the internal mammary nodes are not routinely dissected in patients undergoing breast-conserving therapy or mastectomy with axillary lymph node dissection (ALND).
Experienced surgeons may elect to remove internal mammary nodes if they are identified during SLNB. However, surgeons with less experience removing internal mammary nodes may elect to remove an internal mammary node if it is the only identifiable sentinel node.
When internal mammary nodes are biopsied, tumor involvement is found in approximately 20 percent of patients . Eight to 10 percent of patients without axillary disease are found to have regional metastases to the internal mammary nodes [59,112-117]. Positive internal mammary nodes are most commonly found with medial tumors over 2 cm in size . Tumor involvement of internal mammary nodes is associated with a poor prognosis [110,119]. The diagnosis of positive internal mammary nodes may affect treatment decisions regarding adjuvant systemic therapy and regional nodal irradiation . (See "Radiation therapy techniques for newly diagnosed, non-metastatic breast cancer", section on 'Regional field'.)
Internal mammary nodes are only visualized in approximately 20 percent of patients during SLNB . This is partly explained by limitations of the SLNB technique [45,59,113,114,116,120]. The procedure does not reliably identify involved internal mammary nodes because of interference from radioactivity at the primary tumor site . There is a high rate of technical failure (20 to 39 percent) in patients with parasternal hot spots on lymphoscintigraphy [112,116,117], and hot spots in the internal mammary region do not always represent tumor involvement [59,114,121,122]. In addition, some surgeons do not employ radioactive colloid injection and only rely on blue dye to identify the sentinel nodes.
Accordingly, additional noninvasive methods have been used to assess internal mammary nodes, including magnetic resonance (MR) imaging and positron emission tomography (PET) scanning. Breast MR imaging visualized at least one internal mammary lymph node (range 1 to 3) in 50 of 108 women with an average size of 4.5 mm (range 2 to 9 mm) . However, these imaging modalities are unable to definitively identify positive nodes.
The internal mammary nodes are difficult to sample surgically even when they are identified by lymphoscintigraphy . Although internal mammary lymph node biopsy can be accomplished at the time of mastectomy by splitting the fibers of the pectoralis major muscle, an internal mammary lymph node biopsy in a patient undergoing breast-conserving surgery usually requires a second incision, which is cosmetically visible through many types of clothing. Biopsy of the internal mammary node can be complicated by pneumothorax, pleural effusion, or bleeding and therefore should be performed only when, in the judgment of the surgeon, it can be performed safely [114,116].
Intramammary nodes — Numerous case reports document identification of intramammary lymph nodes on SLNB [124-128], although few have explored the clinical significance of this finding. Intramammary lymph nodes are present in 1 to 28 percent of women with breast cancer. Most series report a high likelihood of additional axillary nodal metastases when the intramammary nodes contain cancer [129,130]. (See "Diagnostic evaluation of women with suspected breast cancer".)
If intramammary nodes contain tumor, they are treated the same as a positive axillary lymph node for staging and prognosing purposes .
INFORMATION FOR PATIENTS — UpToDate offers two types of patient education materials, "The Basics" and "Beyond the Basics." The Basics patient education pieces are written in plain language, at the 5th to 6th grade reading level, and they answer the four or five key questions a patient might have about a given condition. These articles are best for patients who want a general overview and who prefer short, easy-to-read materials. Beyond the Basics patient education pieces are longer, more sophisticated, and more detailed. These articles are written at the 10th to 12th grade reading level and are best for patients who want in-depth information and are comfortable with some medical jargon.
Here are the patient education articles that are relevant to this topic. We encourage you to print or e-mail these topics to your patients. (You can also locate patient education articles on a variety of subjects by searching on "patient info" and the keyword(s) of interest.)
●Basics topic (see "Patient education: Sentinel lymph node biopsy for breast cancer (The Basics)")
●Beyond the Basics topic (see "Patient education: Breast cancer guide to diagnosis and treatment (Beyond the Basics)")
SUMMARY AND RECOMMENDATIONS
●The status of the axillary lymph nodes is one of the most important prognostic factors in patients with breast cancer. Histologic examination of removed lymph nodes is the most accurate method for assessing nodal metastasis. An adequate clinical evaluation of the axilla (which may require imaging in obese patients) is required for treatment decisions in patients with invasive breast cancer, and axillary lymph node dissection (ALND) remains the standard approach for women who are clinically node-positive. (See 'Introduction' above and 'Preoperative axilla evaluation' above.)
●For patients with early-stage breast cancer who are clinically node-negative, we recommend an initial axillary evaluation with sentinel lymph node biopsy (SLNB) rather than ALND (Grade 1A). (See 'Early breast cancer with clinically negative node' above.)
●For patients with ductal carcinoma in situ (DCIS) undergoing breast-conserving surgery, we recommend not performing surgical evaluation of the axilla (Grade 1A). For patients with DCIS undergoing a mastectomy, we suggest that a SLNB be performed, as SLNB is not possible after a mastectomy, even if invasive disease is later found (Grade 2B). (See 'DCIS with planned mastectomy or suspicious features' above.)
●For older patients who are clinically node-negative, in whom the nodal information will not affect adjuvant treatment decisions, we suggest omitting surgical evaluation of the axilla (Grade 2B). (See 'Patients whose axillary status does not guide adjuvant treatment' above.)
●Inflammatory breast cancer is an absolute contraindication to SLNB. The use of sentinel node biopsy in special circumstances, including patients with large tumors, patients undergoing neoadjuvant chemotherapy, pregnant patients, and those who have had prior breast or axillary surgery, remains controversial. (See 'Special circumstances' above.)
●In patients who have recurrent breast cancer and previous axillary procedures (SLNB or ALND), we suggest performing a lymphoscintigraphy using 99mTc-colloidal albumin or sulfur colloid to identify the sentinel node rather than proceeding directly to an SLNB (Grade 2C). (See 'Recurrent breast cancer and previous axillary procedures' above.)
●Immunohistochemical assessment (IHC) should be not be used as a routine method of evaluating sentinel nodes. IHC should be employed to definitively diagnose an area that is suspicious for, but not diagnostic of, lymph node metastases on hematoxylin and eosin (H&E). (See 'Pathologic analysis of nodal metastases' above.)
●The decision to proceed with completion ALND after SLNB is based on the results of the SLNB and whether the patient is "Z-0011-eligible" (defined as clinically node-negative, early-stage [T1/T2, ≤5 cm] breast cancer metastatic to one to two sentinel lymph nodes [without extranodal extension] undergoing breast-conserving surgery followed by whole-breast radiotherapy) (see 'Management after sentinel lymph node biopsy' above):
•For patients who are clinically node-negative and have a SLNB that is negative or shows only isolated tumor cells, we recommend not performing completion ALND (Grade 1A). (See 'No sentinel node metastasis' above.)
•For patients who have a SLNB with one or two positive sentinel nodes and are "Z-0011-eligible," we recommend not performing completion axillary dissection (Grade 1A). If only partial-breast irradiation is planned, completion ALND should be performed. (See 'One or two sentinel node metastases' above.).
•For women who have one or two positive sentinel nodes but are not "Z-0011-eligible" (eg, large [T3, >5 cm] tumor or the presence of extranodal extension), we recommend performing completion ALND or axillary radiation (Grade 1C). (See 'Sentinel node metastasis with large tumor' above and 'Sentinel node metastasis with extranodal extension' above and 'Role of radiotherapy' above.)
- Alkuwari E, Auger M. Accuracy of fine-needle aspiration cytology of axillary lymph nodes in breast cancer patients: a study of 115 cases with cytologic-histologic correlation. Cancer 2008; 114:89.
- Hinson JL, McGrath P, Moore A, et al. The critical role of axillary ultrasound and aspiration biopsy in the management of breast cancer patients with clinically negative axilla. Ann Surg Oncol 2008; 15:250.
- Koelliker SL, Chung MA, Mainiero MB, et al. Axillary lymph nodes: US-guided fine-needle aspiration for initial staging of breast cancer--correlation with primary tumor size. Radiology 2008; 246:81.
- Holwitt DM, Swatske ME, Gillanders WE, et al. Scientific Presentation Award: The combination of axillary ultrasound and ultrasound-guided biopsy is an accurate predictor of axillary stage in clinically node-negative breast cancer patients. Am J Surg 2008; 196:477.
- Britton PD, Goud A, Godward S, et al. Use of ultrasound-guided axillary node core biopsy in staging of early breast cancer. Eur Radiol 2009; 19:561.
- Abe H, Schmidt RA, Kulkarni K, et al. Axillary lymph nodes suspicious for breast cancer metastasis: sampling with US-guided 14-gauge core-needle biopsy--clinical experience in 100 patients. Radiology 2009; 250:41.
- Mills P, Sever A, Weeks J, et al. Axillary ultrasound assessment in primary breast cancer: an audit of 653 cases. Breast J 2010; 16:460.
- van Wely BJ, de Wilt JH, Francissen C, et al. Meta-analysis of ultrasound-guided biopsy of suspicious axillary lymph nodes in the selection of patients with extensive axillary tumour burden in breast cancer. Br J Surg 2015; 102:159.
- Verheuvel NC, van den Hoven I, Ooms HW, et al. The role of ultrasound-guided lymph node biopsy in axillary staging of invasive breast cancer in the post-ACOSOG Z0011 trial era. Ann Surg Oncol 2015; 22:409.
- Lyman GH, Temin S, Edge SB, et al. Sentinel lymph node biopsy for patients with early-stage breast cancer: American Society of Clinical Oncology clinical practice guideline update. J Clin Oncol 2014; 32:1365.
- National Comprehensive Cancer Network (NCCN). NCCN Clinical practice guidelines in oncology. http://www.nccn.org/professionals/physician_gls/f_guidelines.asp (Accessed on February 27, 2016).
- Virnig BA, Tuttle TM, Shamliyan T, Kane RL. Ductal carcinoma in situ of the breast: a systematic review of incidence, treatment, and outcomes. J Natl Cancer Inst 2010; 102:170.
- Brennan ME, Turner RM, Ciatto S, et al. Ductal carcinoma in situ at core-needle biopsy: meta-analysis of underestimation and predictors of invasive breast cancer. Radiology 2011; 260:119.
- Lyman GH, Giuliano AE, Somerfield MR, et al. American Society of Clinical Oncology guideline recommendations for sentinel lymph node biopsy in early-stage breast cancer. J Clin Oncol 2005; 23:7703.
- Weaver DL. Occult "micrometastases" in ductal carcinoma in situ: investigative implications for sentinel lymph node biopsy. Cancer 2003; 98:2083.
- Lara JF, Young SM, Velilla RE, et al. The relevance of occult axillary micrometastasis in ductal carcinoma in situ: a clinicopathologic study with long-term follow-up. Cancer 2003; 98:2105.
- Kaufmann M, Morrow M, von Minckwitz G, et al. Locoregional treatment of primary breast cancer: consensus recommendations from an International Expert Panel. Cancer 2010; 116:1184.
- Chung MH, Ye W, Giuliano AE. Role for sentinel lymph node dissection in the management of large (> or = 5 cm) invasive breast cancer. Ann Surg Oncol 2001; 8:688.
- Wong SL, Chao C, Edwards MJ, et al. Accuracy of sentinel lymph node biopsy for patients with T2 and T3 breast cancers. Am Surg 2001; 67:522.
- Hidar S, Bibi M, Gharbi O, et al. Sentinel lymph node biopsy after neoadjuvant chemotherapy in inflammatory breast cancer. Int J Surg 2009; 7:272.
- Singletary SE. Surgical management of inflammatory breast cancer. Semin Oncol 2008; 35:72.
- Hughes KS, Schnaper LA, Bellon JR, et al. Lumpectomy plus tamoxifen with or without irradiation in women age 70 years or older with early breast cancer: long-term follow-up of CALGB 9343. J Clin Oncol 2013; 31:2382.
- Klimberg VS, Rubio IT, Henry R, et al. Subareolar versus peritumoral injection for location of the sentinel lymph node. Ann Surg 1999; 229:860.
- Borgstein PJ, Meijer S, Pijpers RJ, van Diest PJ. Functional lymphatic anatomy for sentinel node biopsy in breast cancer: echoes from the past and the periareolar blue method. Ann Surg 2000; 232:81.
- McMasters KM, Wong SL, Martin RC 2nd, et al. Dermal injection of radioactive colloid is superior to peritumoral injection for breast cancer sentinel lymph node biopsy: results of a multiinstitutional study. Ann Surg 2001; 233:676.
- Schrenk P, Wayand W. Sentinel-node biopsy in axillary lymph-node staging for patients with multicentric breast cancer. Lancet 2001; 357:122.
- Kern KA. Concordance and validation study of sentinel lymph node biopsy for breast cancer using subareolar injection of blue dye and technetium 99m sulfur colloid. J Am Coll Surg 2002; 195:467.
- Knauer M, Konstantiniuk P, Haid A, et al. Multicentric breast cancer: a new indication for sentinel node biopsy--a multi-institutional validation study. J Clin Oncol 2006; 24:3374.
- Celebioglu F, Frisell J, Danielsson R, Bergkvist L. Sentinel node biopsy in non-palpable breast cancer and in patients with a previous diagnostic excision. Eur J Surg Oncol 2007; 33:276.
- Heuts EM, van der Ent FW, Kengen RA, et al. Results of sentinel node biopsy not affected by previous excisional biopsy. Eur J Surg Oncol 2006; 32:278.
- Intra M, Trifirò G, Viale G, et al. Second biopsy of axillary sentinel lymph node for reappearing breast cancer after previous sentinel lymph node biopsy. Ann Surg Oncol 2005; 12:895.
- Taback B, Nguyen P, Hansen N, et al. Sentinel lymph node biopsy for local recurrence of breast cancer after breast-conserving therapy. Ann Surg Oncol 2006; 13:1099.
- Newman EA, Cimmino VM, Sabel MS, et al. Lymphatic mapping and sentinel lymph node biopsy for patients with local recurrence after breast-conservation therapy. Ann Surg Oncol 2006; 13:52.
- Maaskant-Braat AJ, Voogd AC, Roumen RM, Nieuwenhuijzen GA. Repeat sentinel node biopsy in patients with locally recurrent breast cancer: a systematic review and meta-analysis of the literature. Breast Cancer Res Treat 2013; 138:13.
- Maaskant-Braat AJ, Roumen RM, Voogd AC, et al. Sentinel Node and Recurrent Breast Cancer (SNARB): results of a nationwide registration study. Ann Surg Oncol 2013; 20:620.
- Vugts G, Maaskant-Braat AJ, Voogd AC, et al. Repeat sentinel node biopsy should be considered in patients with locally recurrent breast cancer. Breast Cancer Res Treat 2015; 153:549.
- Ahmed M, Baker R, Rubio IT. Meta-analysis of aberrant lymphatic drainage in recurrent breast cancer. Br J Surg 2016; 103:1579.
- Boughey JC, Bedrosian I, Meric-Bernstam F, et al. Comparative analysis of sentinel lymph node operation in male and female breast cancer patients. J Am Coll Surg 2006; 203:475.
- Lymphazurin (isosulfan blue) injection, soluiton. dailymed.nlm.nih.gov/dailymed/lookup.cfm?setid=cbee4532-238b-4f91-86ae-f8c0c1b6d7da (Accessed on July 24, 2014).
- Gropper AB, Calvillo KZ, Dominici L, et al. Sentinel lymph node biopsy in pregnant women with breast cancer. Ann Surg Oncol 2014; 21:2506.
- Gentilini O, Cremonesi M, Trifirò G, et al. Safety of sentinel node biopsy in pregnant patients with breast cancer. Ann Oncol 2004; 15:1348.
- Cragan JD. Teratogen update: methylene blue. Teratology 1999; 60:42.
- Khera SY, Kiluk JV, Hasson DM, et al. Pregnancy-associated breast cancer patients can safely undergo lymphatic mapping. Breast J 2008; 14:250.
- Ban EJ, Lee JS, Koo JS, et al. How many sentinel lymph nodes are enough for accurate axillary staging in t1-2 breast cancer? J Breast Cancer 2011; 14:296.
- Albertini JJ, Lyman GH, Cox C, et al. Lymphatic mapping and sentinel node biopsy in the patient with breast cancer. JAMA 1996; 276:1818.
- Borgstein PJ, Pijpers R, Comans EF, et al. Sentinel lymph node biopsy in breast cancer: guidelines and pitfalls of lymphoscintigraphy and gamma probe detection. J Am Coll Surg 1998; 186:275.
- Krag DN, Weaver DL, Alex JC, Fairbank JT. Surgical resection and radiolocalization of the sentinel lymph node in breast cancer using a gamma probe. Surg Oncol 1993; 2:335.
- Giuliano AE, Kirgan DM, Guenther JM, Morton DL. Lymphatic mapping and sentinel lymphadenectomy for breast cancer. Ann Surg 1994; 220:391.
- Giuliano AE, Morrow M, Duggal S, Julian TB. Should ACOSOG Z0011 change practice with respect to axillary lymph node dissection for a positive sentinel lymph node biopsy in breast cancer? Clin Exp Metastasis 2012; 29:687.
- Fisher B, Jeong JH, Anderson S, et al. Twenty-five-year follow-up of a randomized trial comparing radical mastectomy, total mastectomy, and total mastectomy followed by irradiation. N Engl J Med 2002; 347:567.
- Giuliano AE, McCall L, Beitsch P, et al. Locoregional recurrence after sentinel lymph node dissection with or without axillary dissection in patients with sentinel lymph node metastases: the American College of Surgeons Oncology Group Z0011 randomized trial. Ann Surg 2010; 252:426.
- Latosinsky S, Berrang TS, Cutter CS, et al. CAGS and ACS Evidence Based Reviews in Surgery. 40. Axillary dissection versus no axillary dissection in women with invasive breast cancer and sentinel node metastasis. Can J Surg 2012; 55:66.
- Galimberti V, Cole BF, Zurrida S, et al. Axillary dissection versus no axillary dissection in patients with sentinel-node micrometastases (IBCSG 23-01): a phase 3 randomised controlled trial. Lancet Oncol 2013; 14:297.
- Donker M, van Tienhoven G, Straver ME, et al. Radiotherapy or surgery of the axilla after a positive sentinel node in breast cancer (EORTC 10981-22023 AMAROS): a randomised, multicentre, open-label, phase 3 non-inferiority trial. Lancet Oncol 2014; 15:1303.
- Rao R, Euhus D, Mayo HG, Balch C. Axillary node interventions in breast cancer: a systematic review. JAMA 2013; 310:1385.
- Shimazu K, Tamaki Y, Taguchi T, et al. Lymphoscintigraphic visualization of internal mammary nodes with subtumoral injection of radiocolloid in patients with breast cancer. Ann Surg 2003; 237:390.
- Tanis PJ, Deurloo EE, Valdés Olmos RA, et al. Single intralesional tracer dose for radio-guided excision of clinically occult breast cancer and sentinel node. Ann Surg Oncol 2001; 8:850.
- Doting MH, Jansen L, Nieweg OE, et al. Lymphatic mapping with intralesional tracer administration in breast carcinoma patients. Cancer 2000; 88:2546.
- Harlow S, Krag D, Weaver D, Ashikaga T. Extra-Axillary Sentinel Lymph Nodes in Breast Cancer. Breast Cancer 1999; 6:159.
- Breast Section of the Royal Australasian College of Surgeons: sentinel node biopsy in breast cancer 2005. www.nbcc.org.au/media/Sentinel_node_biopsy.html (Accessed on November 05, 2008).
- Schwartz GF, Giuliano AE, Veronesi U, Consensus Conference Committee. Proceedings of the consensus conference on the role of sentinel lymph node biopsy in carcinoma of the breast, April 19-22, 2001, Philadelphia, Pennsylvania. Cancer 2002; 94:2542.
- Cantin J, Scarth H, Levine M, et al. Clinical practice guidelines for the care and treatment of breast cancer: 13. Sentinel lymph node biopsy. CMAJ 2001; 165:166.
- Cserni G, Bianchi S, Vezzosi V, et al. The value of cytokeratin immunohistochemistry in the evaluation of axillary sentinel lymph nodes in patients with lobular breast carcinoma. J Clin Pathol 2006; 59:518.
- Weinberg ES, Dickson D, White L, et al. Cytokeratin staining for intraoperative evaluation of sentinel lymph nodes in patients with invasive lobular carcinoma. Am J Surg 2004; 188:419.
- American Joint Committee on Cancer. Cancer Staging Manual, 7th ed, Edge SB (Ed), Springer, New York 2010. p.347.
- Carter BA, Page DL. Sentinel lymph node histopathology in breast cancer: minimal disease versus artifact. J Clin Oncol 2006; 24:1978.
- Youngson BJ, Liberman L, Rosen PP. Displacement of carcinomatous epithelium in surgical breast specimens following stereotaxic core biopsy. Am J Clin Pathol 1995; 103:598.
- Douglas-Jones AG, Verghese A. Diagnostic difficulty arising from displaced epithelium after core biopsy in intracystic papillary lesions of the breast. J Clin Pathol 2002; 55:780.
- Moore KH, Thaler HT, Tan LK, et al. Immunohistochemically detected tumor cells in the sentinel lymph nodes of patients with breast carcinoma: biologic metastasis or procedural artifact? Cancer 2004; 100:929.
- Weaver DL, Ashikaga T, Krag DN, et al. Effect of occult metastases on survival in node-negative breast cancer. N Engl J Med 2011; 364:412.
- Gooch J, King TA, Eaton A, et al. The extent of extracapsular extension may influence the need for axillary lymph node dissection in patients with T1-T2 breast cancer. Ann Surg Oncol 2014; 21:2897.
- Weaver DL, Krag DN, Ashikaga T, et al. Pathologic analysis of sentinel and nonsentinel lymph nodes in breast carcinoma: a multicenter study. Cancer 2000; 88:1099.
- Giuliano AE, Hawes D, Ballman KV, et al. Association of occult metastases in sentinel lymph nodes and bone marrow with survival among women with early-stage invasive breast cancer. JAMA 2011; 306:385.
- Krag DN, Anderson SJ, Julian TB, et al. Technical outcomes of sentinel-lymph-node resection and conventional axillary-lymph-node dissection in patients with clinically node-negative breast cancer: results from the NSABP B-32 randomised phase III trial. Lancet Oncol 2007; 8:881.
- Krag DN, Julian TB, Harlow SP, et al. NSABP-32: Phase III, randomized trial comparing axillary resection with sentinal lymph node dissection: a description of the trial. Ann Surg Oncol 2004; 11:208S.
- Primary outcome results of NSABP B-32, a randomized phase III clinical trial to compare sentinel node resection (SNR) to conventional axillary dissection (AD) in clinically node-negative breast cancer patients. ASCO 2010; Abstract LBA505. J Clin Oncol 2010; 28:18s (suppl; abstr LBA505).
- Krag DN, Anderson SJ, Julian TB, et al. Sentinel-lymph-node resection compared with conventional axillary-lymph-node dissection in clinically node-negative patients with breast cancer: overall survival findings from the NSABP B-32 randomised phase 3 trial. Lancet Oncol 2010; 11:927.
- Reed J, Rosman M, Verbanac KM, et al. Prognostic implications of isolated tumor cells and micrometastases in sentinel nodes of patients with invasive breast cancer: 10-year analysis of patients enrolled in the prospective East Carolina University/Anne Arundel Medical Center Sentinel Node Multicenter Study. J Am Coll Surg 2009; 208:333.
- Chagpar A, Middleton LP, Sahin AA, et al. Clinical outcome of patients with lymph node-negative breast carcinoma who have sentinel lymph node micrometastases detected by immunohistochemistry. Cancer 2005; 103:1581.
- Pugliese MS, Beatty JD, Tickman RJ, et al. Impact and outcomes of routine microstaging of sentinel lymph nodes in breast cancer: significance of the pN0(i+) and pN1mi categories. Ann Surg Oncol 2009; 16:113.
- Kimbrough CW, McMasters KM, Quillo A, Ajkay N. Occult metastases in node-negative breast cancer: A Surveillance, Epidemiology, and End Results-based analysis. Surgery 2015; 158:494.
- Krag D, Weaver D, Ashikaga T, et al. The sentinel node in breast cancer--a multicenter validation study. N Engl J Med 1998; 339:941.
- Giuliano AE, Jones RC, Brennan M, Statman R. Sentinel lymphadenectomy in breast cancer. J Clin Oncol 1997; 15:2345.
- Veronesi U, Paganelli G, Galimberti V, et al. Sentinel-node biopsy to avoid axillary dissection in breast cancer with clinically negative lymph-nodes. Lancet 1997; 349:1864.
- Turner RR, Chu KU, Qi K, et al. Pathologic features associated with nonsentinel lymph node metastases in patients with metastatic breast carcinoma in a sentinel lymph node. Cancer 2000; 89:574.
- Lyman GH, Kim TY, Giuliano AE. A systematic review and meta-analysis of lymphatic mapping and sentinel node biopsy (SNB) in early-stage breast cancer (ESBC) (abstract 2001). Data presented at the 27th Annual San Antonio Breast Cancer Symposium, San Antonio, TX, December 9, 2004.
- Giuliano AE, Haigh PI, Brennan MB, et al. Prospective observational study of sentinel lymphadenectomy without further axillary dissection in patients with sentinel node-negative breast cancer. J Clin Oncol 2000; 18:2553.
- Katz A, Niemierko A, Gage I, et al. Can axillary dissection be avoided in patients with sentinel lymph node metastasis? J Surg Oncol 2006; 93:550.
- Dauphine CE, Haukoos JS, Vargas MP, et al. Evaluation of three scoring systems predicting non sentinel node metastasis in breast cancer patients with a positive sentinel node biopsy. Ann Surg Oncol 2007; 14:1014.
- Gur AS, Unal B, Johnson R, et al. Predictive probability of four different breast cancer nomograms for nonsentinel axillary lymph node metastasis in positive sentinel node biopsy. J Am Coll Surg 2009; 208:229.
- Van Zee KJ, Manasseh DM, Bevilacqua JL, et al. A nomogram for predicting the likelihood of additional nodal metastases in breast cancer patients with a positive sentinel node biopsy. Ann Surg Oncol 2003; 10:1140.
- Lambert LA, Ayers GD, Hwang RF, et al. Validation of a breast cancer nomogram for predicting nonsentinel lymph node metastases after a positive sentinel node biopsy. Ann Surg Oncol 2006; 13:310.
- Katz A, Smith BL, Golshan M, et al. Nomogram for the prediction of having four or more involved nodes for sentinel lymph node-positive breast cancer. J Clin Oncol 2008; 26:2093.
- Mittendorf EA, Hunt KK, Boughey JC, et al. Incorporation of sentinel lymph node metastasis size into a nomogram predicting nonsentinel lymph node involvement in breast cancer patients with a positive sentinel lymph node. Ann Surg 2012; 255:109.
- van den Hoven I, Kuijt G, Roumen R, et al. A head to head comparison of nine tools predicting non-sentinel lymph node status in sentinel node positive breast cancer women. J Surg Oncol 2015; 112:133.
- Lucci A, McCall LM, Beitsch PD, et al. Surgical complications associated with sentinel lymph node dissection (SLND) plus axillary lymph node dissection compared with SLND alone in the American College of Surgeons Oncology Group Trial Z0011. J Clin Oncol 2007; 25:3657.
- Giuliano AE, Hunt KK, Ballman KV, et al. Axillary dissection vs no axillary dissection in women with invasive breast cancer and sentinel node metastasis: a randomized clinical trial. JAMA 2011; 305:569.
- Giuliano AE, Ballman K, McCall L, et al. Locoregional Recurrence After Sentinel Lymph Node Dissection With or Without Axillary Dissection in Patients With Sentinel Lymph Node Metastases: Long-term Follow-up From the American College of Surgeons Oncology Group (Alliance) ACOSOG Z0011 Randomized Trial. Ann Surg 2016; 264:413.
- Choi AH, Surrusco M, Rodriguez S, et al. Extranodal extension on sentinel lymph node dissection: why should we treat it differently? Am Surg 2014; 80:932.
- Changsri C, Prakash S, Sandweiss L, Bose S. Prediction of additional axillary metastasis of breast cancer following sentinel lymph node surgery. Breast J 2004; 10:392.
- Abdessalam SF, Zervos EE, Prasad M, et al. Predictors of positive axillary lymph nodes after sentinel lymph node biopsy in breast cancer. Am J Surg 2001; 182:316.
- Choi AH, Blount S, Perez MN, et al. Size of Extranodal Extension on Sentinel Lymph Node Dissection in the American College of Surgeons Oncology Group Z0011 Trial Era. JAMA Surg 2015; 150:1141.
- Stranzl H, Ofner P, Peintinger F. Postoperative irradiation in breast cancer patients with one to three positive axillary lymph nodes. Is there an impact of axillary extranodal tumor extension on locoregional and distant control? Strahlenther Onkol 2006; 182:583.
- Neri A, Marrelli D, Roviello F, et al. Prognostic value of extracapsular extension of axillary lymph node metastases in T1 to T3 breast cancer. Ann Surg Oncol 2005; 12:246.
- Ilknur GB, Hilmi A, Tülay C, et al. The importance of extracapsular extension of axillary lymph node metastases in breast cancer. Tumori 2004; 90:107.
- Altinyollar H, Berberoğlu U, Gülben K, Irkin F. The correlation of extranodal invasion with other prognostic parameters in lymph node positive breast cancer. J Surg Oncol 2007; 95:567.
- Bucci JA, Kennedy CW, Burn J, et al. Implications of extranodal spread in node positive breast cancer: a review of survival and local recurrence. Breast 2001; 10:213.
- Drinka E, Allen P, McBride A, et al. Metastatic Tumor Volume and Extranodal Tumor Extension: Clinical Significance in Patients With Stage II Breast Cancer. Arch Pathol Lab Med 2015; 139:1288.
- Jagsi R, Chadha M, Moni J, et al. Radiation field design in the ACOSOG Z0011 (Alliance) Trial. J Clin Oncol 2014; 32:3600.
- Cody HS 3rd, Urban JA. Internal mammary node status: a major prognosticator in axillary node-negative breast cancer. Ann Surg Oncol 1995; 2:32.
- Veronesi U, Marubini E, Mariani L, et al. The dissection of internal mammary nodes does not improve the survival of breast cancer patients. 30-year results of a randomised trial. Eur J Cancer 1999; 35:1320.
- Heuts EM, van der Ent FW, von Meyenfeldt MF, Voogd AC. Internal mammary lymph drainage and sentinel node biopsy in breast cancer - A study on 1008 patients. Eur J Surg Oncol 2009; 35:252.
- Chen RC, Lin NU, Golshan M, et al. Internal mammary nodes in breast cancer: diagnosis and implications for patient management -- a systematic review. J Clin Oncol 2008; 26:4981.
- Mansel RE, Goyal A, Newcombe RG, ALMANAC Trialists Group. Internal mammary node drainage and its role in sentinel lymph node biopsy: the initial ALMANAC experience. Clin Breast Cancer 2004; 5:279.
- Klauber-DeMore N, Bevilacqua JL, Van Zee KJ, et al. Comprehensive review of the management of internal mammary lymph node metastases in breast cancer. J Am Coll Surg 2001; 193:547.
- van der Ent FW, Kengen RA, van der Pol HA, et al. Halsted revisited: internal mammary sentinel lymph node biopsy in breast cancer. Ann Surg 2001; 234:79.
- Tanis PJ, Nieweg OE, Valdés Olmos RA, et al. Impact of non-axillary sentinel node biopsy on staging and treatment of breast cancer patients. Br J Cancer 2002; 87:705.
- Shahar KH, Buchholz TA, Delpassand E, et al. Lower and central tumor location correlates with lymphoscintigraphy drainage to the internal mammary lymph nodes in breast carcinoma. Cancer 2005; 103:1323.
- Donegan WL. The influence of untreated internal mammary metastases upon the course of mammary cancer. Cancer 1977; 39:533.
- Noguchi M, Tsugawa K, Miwa K. Internal mammary chain sentinel lymph node identification in breast cancer. J Surg Oncol 2000; 73:75.
- Fabry HF, Mutsaers PG, Meijer S, et al. Clinical relevance of parasternal uptake in sentinel node procedure for breast cancer. J Surg Oncol 2004; 87:13.
- Carcoforo P, Sortini D, Feggi L, et al. Clinical and therapeutic importance of sentinel node biopsy of the internal mammary chain in patients with breast cancer: a single-center study with long-term follow-up. Ann Surg Oncol 2006; 13:1338.
- Mack M, Chetlen A, Liao J. Incidental Internal Mammary Lymph Nodes Visualized on Screening Breast MRI. AJR Am J Roentgenol 2015; 205:209.
- Gajdos C, Bleiweiss IJ, Drossman S, Tartter PI. Breast cancer in an intramammary sentinel node. Breast J 2001; 7:260.
- Tytler I, Hayes A, Kissin M. Intramammary sentinel nodes in early breast cancer: can we find them and do they matter? Eur J Surg Oncol 2003; 29:6.
- Jansen L, Doting MH, Rutgers EJ, et al. Clinical relevance of sentinel lymph nodes outside the axilla in patients with breast cancer. Br J Surg 2000; 87:920.
- Upponi S, Kalra S, Poultsidis A, et al. The significance of intramammary nodes in primary breast cancer. Eur J Surg Oncol 2001; 27:707.
- Rull M, Fraile M, Alastrué A, et al. Histologically invaded intramammary sentinel node, but no metastases found on axillary dissection. Eur J Surg 1999; 165:1100.
- Intra M, Garcia-Etienne CA, Renne G, et al. When sentinel lymph node is intramammary. Ann Surg Oncol 2008; 15:1304.
- Pugliese MS, Stempel MM, Cody HS 3rd, et al. Surgical management of the axilla: do intramammary nodes matter? Am J Surg 2009; 198:532.
- Shen J, Hunt KK, Mirza NQ, et al. Intramammary lymph node metastases are an independent predictor of poor outcome in patients with breast carcinoma. Cancer 2004; 101:1330.