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Medline ® Abstract for Reference 30

of 'Molecular genetics of colorectal cancer'

Altered growth of human colon cancer cell lines disrupted at activated Ki-ras.
Shirasawa S, Furuse M, Yokoyama N, Sasazuki T
Science. 1993;260(5104):85.
Point mutations that activate the Ki-ras proto-oncogene are presented in about 50 percent of human colorectal tumors. To study the functional significance of these mutations, the activated Ki-ras genes in two human colon carcinoma cell lines, DLD-1 and HCT 116, were disrupted by homologous recombination. Compared with parental cells, cells disrupted at the activated Ki-ras gene were morphologically altered, lost the capacity for anchorage-independent growth, grew more slowly both in vitro and in nude mice, and showed reduced expression of c-myc. Thus, the activated Ki-ras gene plays a key role in colorectal tumorigenesis through altered cell differentiation and cell growth.
Department of Genetics, Kyushu University, Fukuoka, Japan.