Analysis of MC(T) and MC(TC) mast cells in tissue

Methods Mol Biol. 2006:315:53-62.

Abstract

The MC(TC) and MCT types of human mast cells initially were recognized on the basis of the protease compositions of their secretory granules, with tryptase, chymase, carboxypeptidase A3, and cathepsin G in the former and only tryptase in the latter. Antibodies against chymase and tryptase traditionally have been used to distinguish these mast cell types from one another. Antitryptase antibodies label all mast cells; antichymase labels only the MC(TC) type. To identify both types in a tissue section, a sequential double-labeling scheme was developed to first stain chymase-positive cells, thereby blocking their recognition by the antitryptase antibody, which will label only the chymase-negative mast cells. In general, these immunocytochemical techniques are more sensitive and specific than classical histochemical techniques for detecting mast cells.

MeSH terms

  • Biomarkers / analysis
  • Cell Separation / methods*
  • Chymases
  • Humans
  • Immunohistochemistry / methods
  • Mast Cells / chemistry
  • Mast Cells / enzymology*
  • Mast Cells / immunology
  • Mast Cells / ultrastructure
  • Serine Endopeptidases / chemistry
  • Serine Endopeptidases / metabolism
  • Tryptases

Substances

  • Biomarkers
  • Serine Endopeptidases
  • Chymases
  • Tryptases