Function and clinical applications of immunoglobulins
- Francisco A Bonilla, MD, PhD
Francisco A Bonilla, MD, PhD
- Section Editor — Immunology and Immunodeficiency
- Associate Professor of Pediatrics
- Harvard Medical School
Immunoglobulin (Ig) molecules are multifunctional components of the immune system that mediate interactions between antigen molecules and a variety of cellular and humoral effector mechanisms. Important mechanisms in which Ig molecules are essential include the following:
●Activation and signal transduction in B cells (in the case of surface Ig receptors)
●Interactions with receptors for the constant region of IgG on a variety of cells, with different functional outcomes
●Activation and modulation of the complement system
Different parts of immunoglobulin molecules have distinct functions. These functional fragments can be generated by digestion with the protease papain, which cleaves immunoglobulins near the hinge region, yielding two identical Fab fragments, and one Fc fragment (figure 1) :
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- FUNCTIONS OF THE Ig VARIABLE REGION
- Antibody-antigen interactions
- Antibody specificity and cross-reactivity
- - Natural antibodies
- - Diagnostic applications
- - Immunohistochemistry
- - Coombs' test
- FUNCTIONS OF THE Ig CONSTANT REGION
- Complement fixation
- Interaction with Fc receptors
- - Opsonization
- THERAPEUTIC APPLICATIONS OF IMMUNOGLOBULINS
- Polyclonal antibodies
- Monoclonal antibodies
- - Production techniques
- - Clinical use
- - Problems
- Modified monoclonal antibodies
- - Humanized monoclonal antibodies
- - Antibody Fab fragments
- - Bifunctional antibodies
- - Antigenized antibodies
- IgG1 fusion proteins
- Monoclonal antibody nomenclature