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Cervical cancer screening tests: Techniques for cervical cytology and human papillomavirus testing

Sarah Feldman, MD, MPH
Christopher P Crum, MD
Section Editor
Barbara Goff, MD
Deputy Editor
Sandy J Falk, MD, FACOG


Cervical cancer screening detects preinvasive neoplasia, thereby making treatment possible before the disease becomes invasive; invasive disease may also be detected. Screening is performed using cervical cytology (Pap test) or a human papillomavirus (HPV) test, or a combination of the two tests.

Techniques for obtaining specimens for cervical cytology and HPV testing are reviewed here. Screening strategies and interpretation of the cervical cytology report are discussed separately. (See "Screening for cervical cancer" and "Cervical and vaginal cytology: Interpretation of results (Pap test report)".)


Cell samples for cervical cytology and human papillomavirus (HPV) testing are obtained during the speculum examination. With certain types of Pap tests, the same specimen can be used for both tests; alternatively, separate specimens can be obtained.

Specimens for cytology — There are two methods for preparing a specimen for cervical cytology: the conventional Pap smear and the liquid-based, thin layer preparation.

For both methods, cells are obtained from the external surface of the cervix (ectocervix) and the cervical canal (endocervix) to evaluate the transformation zone (squamocolumnar junction), the area at greatest risk for neoplasia.


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Literature review current through: Sep 2016. | This topic last updated: Sep 4, 2015.
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