Medline ® Abstract for Reference 52
of 'Cellular and molecular biology of chronic myeloid leukemia'
Functional role for the c-Abl tyrosine kinase in meiosis I.
Kharbanda S, Pandey P, Morris PL, Whang Y, Xu Y, Sawant S, Zhu LJ, Kumar N, Yuan ZM, Weichselbaum R, Sawyers CL, Pandita TK, Kufe D
The c-Abl tyrosine kinase is activated by ionizing radiation and certain other DNA-damaging agents. The DNA-dependent protein kinase (DNA-PK) and the ataxia telangiectasia mutated (ATM) gene product, effectors in the DNA damage response, contribute to the induction of c-Abl activity. The present study demonstrates that c-Abl is expressed in mouse and rat testes, and predominantly in pachytene spermatocytes of meiosis I. The results also demonstrate that c-Abl interacts directly with meiotic chromosomes. In concert with a requirement for c-Abl at the pachytene stage, we show that, in contrast to wild-type mice, testes from Abl-/- mice exhibit defects in spermatogenesis. These findings provide the first demonstration that c-Abl plays a functional role in meiosis.
Cancer Pharmacology, Dana-Farber Cancer Institute, Harvard Medical School, Boston, Massachusetts 02115, USA.