Abstract
The breast/ovarian cancer susceptibility gene BRCA1 exerts its tumor suppressor function, at least in part, by participating in DNA repair and/or DNA damage-responsive pathways. BRCA1 protein is hyperphosphorylated following various DNA-damaging events. Here, we report that the ataxia telangiectasia mutated protein-related protein kinase (ATR) is involved in the phosphorylation of BRCA1 following gamma radiation and hydroxyurea treatment. We have shown that ATR can phosphorylate several BRCA1 fragments in vitro and that a kinase-inactive mutant of ATR interacts with BRCA1 in vivo. Taken together, these results suggest that ATR directly phosphorylates BRCA1 following DNA damage.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Androstadienes / pharmacology
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Antineoplastic Agents / pharmacology
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Ataxia Telangiectasia Mutated Proteins
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BRCA1 Protein / metabolism*
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Breast Neoplasms
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Cell Cycle Proteins / metabolism*
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DNA Damage / physiology*
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DNA, Neoplasm / radiation effects
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DNA-Binding Proteins
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Enzyme Inhibitors / pharmacology
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Humans
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Hydroxyurea / pharmacology
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Phosphatidylinositol 3-Kinases / metabolism
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Phosphoinositide-3 Kinase Inhibitors
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Phosphorylation
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Protein Serine-Threonine Kinases / metabolism
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Tumor Cells, Cultured
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Tumor Suppressor Proteins
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Wortmannin
Substances
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Androstadienes
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Antineoplastic Agents
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BRCA1 Protein
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Cell Cycle Proteins
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DNA, Neoplasm
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DNA-Binding Proteins
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Enzyme Inhibitors
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Phosphoinositide-3 Kinase Inhibitors
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Tumor Suppressor Proteins
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ATM protein, human
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ATR protein, human
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Ataxia Telangiectasia Mutated Proteins
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Protein Serine-Threonine Kinases
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Hydroxyurea
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Wortmannin