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Assessment of fetal lung maturity

INTRODUCTION

The pulmonary system is among the last of the fetal organ systems to mature, both functionally and structurally. Race may affect the timing of fetal lung maturation: fetal lung maturity appears to be achieved earlier in gestation in blacks and South Asians [1].

Because the immature pulmonary system may not oxygenate the neonate adequately, preterm birth can lead to significant neonatal morbidity or mortality [2,3]. Therefore, fetal pulmonary maturity is sometimes assessed before iatrogenic preterm delivery and can be a factor in determining the timing of delivery in these cases. Several laboratory tests are available for this purpose [4]. All involve testing amniotic fluid, which is an indirect assessment of the likelihood of lung maturity; direct studies of fetal pulmonary function are not possible.

This topic will discuss tests for assessment of fetal lung maturity. Clinical manifestations, diagnosis, treatment, sequelae, and prevention of neonatal pulmonary immaturity are reviewed separately. (See "Pathophysiology and clinical manifestations of respiratory distress syndrome in the newborn" and "Prevention and treatment of respiratory distress syndrome in preterm infants" and "Antenatal corticosteroid therapy for reduction of neonatal morbidity and mortality from preterm delivery".)

WHEN IS FETAL PULMONARY MATURITY TESTING PERFORMED?

Term — Elective term cesarean delivery should be scheduled for ≥39 weeks of gestation [5]. Fetal pulmonary maturity can be inferred if:

Review of the patient's prenatal record confirms a gestational age of ≥39 weeks of gestation. (See "Prenatal assessment of gestational age and estimated date of delivery".)

or

                  

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Literature review current through: Jun 2014. | This topic last updated: Feb 11, 2014.
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References
Top
  1. Balchin I, Whittaker JC, Lamont RF, Steer PJ. Timing of planned cesarean delivery by racial group. Obstet Gynecol 2008; 111:659.
  2. Preterm Birth, 1988-1998. National Center for Health Statistics. March of Dimes Perinatal Data Center, 2000.
  3. Parilla BV, Dooley SL, Jansen RD, Socol ML. Iatrogenic respiratory distress syndrome following elective repeat cesarean delivery. Obstet Gynecol 1993; 81:392.
  4. Dubin SB. The laboratory assessment of fetal lung maturity. Am J Clin Pathol 1992; 97:836.
  5. American College of Obstetricians and Gynecologists and American Academy of Pediatrics. Guidelines For Perinatal Care, 7th ed, 2012.
  6. Bates E, Rouse DJ, Mann ML, et al. Neonatal outcomes after demonstrated fetal lung maturity before 39 weeks of gestation. Obstet Gynecol 2010; 116:1288.
  7. American College of Obstetricians and Gynecologists. ACOG Practice Bulletin No. 97: Fetal lung maturity. Obstet Gynecol 2008; 112:717.
  8. Grenache DG, Wilson AR, Gross GA, Gronowski AM. Clinical and laboratory trends in fetal lung maturity testing. Clin Chim Acta 2010; 411:1746.
  9. Neerhof MG, Haney EI, Silver RK, et al. Lamellar body counts compared with traditional phospholipid analysis as an assay for evaluating fetal lung maturity. Obstet Gynecol 2001; 97:305.
  10. Winn-McMillan T, Karon BS. Comparison of the TDx-FLM II and lecithin to sphingomyelin ratio assays in predicting fetal lung maturity. Am J Obstet Gynecol 2005; 193:778.
  11. Haymond S, Luzzi VI, Parvin CA, Gronowski AM. A direct comparison between lamellar body counts and fluorescent polarization methods for predicting respiratory distress syndrome. Am J Clin Pathol 2006; 126:894.
  12. Karcher R, Sykes E, Batton D, et al. Gestational age-specific predicted risk of neonatal respiratory distress syndrome using lamellar body count and surfactant-to-albumin ratio in amniotic fluid. Am J Obstet Gynecol 2005; 193:1680.
  13. Neerhof MG, Dohnal JC, Ashwood ER, et al. Lamellar body counts: a consensus on protocol. Obstet Gynecol 2001; 97:318.
  14. Ventolini G, Neiger R, Hood DL, Belcastro MR. Changes in the threshold of fetal lung maturity testing and neonatal outcome of infants delivered electively before 39 weeks gestation: implications and cost-effectiveness. J Perinatol 2006; 26:264.
  15. Khazardoost S, Yahyazadeh H, Borna S, et al. Amniotic fluid lamellar body count and its sensitivity and specificity in evaluating of fetal lung maturity. J Obstet Gynaecol 2005; 25:257.
  16. Dalence CR, Bowie LJ, Dohnal JC, et al. Amniotic fluid lamellar body count: a rapid and reliable fetal lung maturity test. Obstet Gynecol 1995; 86:235.
  17. Ghidini A, Poggi SH, Spong CY, et al. Role of lamellar body count for the prediction of neonatal respiratory distress syndrome in non-diabetic pregnant women. Arch Gynecol Obstet 2005; 271:325.
  18. Janicki MB, Dries LM, Egan JF, Zelop CM. Determining a cutoff for fetal lung maturity with lamellar body count testing. J Matern Fetal Neonatal Med 2009; 22:419.
  19. Wijnberger LD, Huisjes AJ, Voorbij HA, et al. The accuracy of lamellar body count and lecithin/sphingomyelin ratio in the prediction of neonatal respiratory distress syndrome: a meta-analysis. BJOG 2001; 108:583.
  20. Gluck L, Kulovich MV, Borer RC Jr, et al. Diagnosis of the respiratory distress syndrome by amniocentesis. Am J Obstet Gynecol 1971; 109:440.
  21. Ashwood ER. Standards of laboratory practice: evaluation of fetal lung maturity. National Academy of Clinical Biochemistry. Clin Chem 1997; 43:211.
  22. Whitsett JA, Pryhuber GS, Rice WR. Acute respiratory disorders. In: Neonatology pathophysiology and the management of the newborn, Avery GB, Fletcher MA, MacDonald MG (Eds), JB Lippincott, Philadelphia 1994. p.429.
  23. Gluck L, Kulovich MV, Borer RC Jr, Keidel WN. The interpretation and significance of the lecithin-sphingomyelin ratio in amniotic fluid. Am J Obstet Gynecol 1974; 120:142.
  24. Hallman M, Kulovich M, Kirkpatrick E, et al. Phosphatidylinositol and phosphatidylglycerol in amniotic fluid: indices of lung maturity. Am J Obstet Gynecol 1976; 125:613.
  25. Coapman-Hankin RA, Kiechle FL, Epstein E, et al. Three methods compared for determining phosphatidylglycerol in amniotic fluid. Clin Chem 1985; 31:1374.
  26. Halvorsen PR, Gross TL. Laboratory and clinical evaluation of a rapid slide agglutination test for phosphatidylglycerol. Am J Obstet Gynecol 1985; 151:1061.
  27. Eisenbrey AB, Epstein E, Zak B, et al. Phosphatidylglycerol in amniotic fluid. Comparison of an "ultrasensitive" immunologic assay with TLC and enzymatic assay. Am J Clin Pathol 1989; 91:293.
  28. Lockitch G, Wittmann BK, Mura SM, Hawkley LC. Evaluation of the Amniostat-FLM assay for assessment of fetal lung maturity. Clin Chem 1984; 30:1233.
  29. Knight JA, Miya T, Wu JT. Standard lecithin/sphingomyelin and phosphatidylglycerol techniques compared with immunologic slide test. Obstet Gynecol 1985; 65:840.
  30. Bernstein LH, Stiller R, Menzies C, et al. Amniotic fluid polarization of fluorescence and lecithin/sphingomyelin ratio decision criteria assessed. Yale J Biol Med 1995; 68:101.
  31. Standards of Laboratory Practice: Guidelines for the Evaluation and Management of the Newborn. National Acadamy of Clinical Biochemisry, 1998.
  32. Kesselman EJ, Figueroa R, Garry D, Maulik D. The usefulness of the TDx/TDxFLx fetal lung maturity II assay in the initial evaluation of fetal lung maturity. Am J Obstet Gynecol 2003; 188:1220.
  33. Hagen E, Link JC, Arias F. A comparison of the accuracy of the TDx-FLM assay, lecithin-sphingomyelin ratio, and phosphatidylglycerol in the prediction of neonatal respiratory distress syndrome. Obstet Gynecol 1993; 82:1004.
  34. Clements JA, Platzker AC, Tierney DF, et al. Assessment of the risk of the respiratory-distress syndrome by a rapid test for surfactant in amniotic fluid. N Engl J Med 1972; 286:1077.
  35. Sher G, Statland BE, Freer DE, Kraybill EN. Assessing fetal lung maturation by the foam stability index test. Obstet Gynecol 1978; 52:673.
  36. Jobe AH. Fetal lung development, tests for maturation, induction of maturation and treatment. In: Maternal-Fetal Medicine, Creasy RK, Resnik R (Eds), WB Saunders, Philadelphia 1999. p.418.
  37. Parvin CA, Kaplan LA, Chapman JF, et al. Predicting respiratory distress syndrome using gestational age and fetal lung maturity by fluorescent polarization. Am J Obstet Gynecol 2005; 192:199.
  38. Kaplan LA, Chapman JF, Bock JL, et al. Prediction of respiratory distress syndrome using the Abbott FLM-II amniotic fluid assay. Clin Chim Acta 2002; 326:61.
  39. Tanasijevic MJ, Wybenga DR, Richardson D, et al. A predictive model for fetal lung maturity employing gestational age and test results. Am J Clin Pathol 1994; 102:788.
  40. Gillen-Goldstein J, Kaplan LA, Chapman JF. Prediction of Respiratory Distress Syndrome from Amniotic Fluid Surfactant Measures: The NACB Fetal Lung Maturity Project, Journal of Society for Gynecologic Investigation, 2001. p.257.
  41. Kamath BD, Marcotte MP, DeFranco EA. Neonatal morbidity after documented fetal lung maturity in late preterm and early term infants. Am J Obstet Gynecol 2011; 204:518.e1.
  42. Carlan SJ, Gearity D, O'Brien WF. The effect of maternal blood contamination on the TDx-FLM II assay. Am J Perinatol 1997; 14:491.
  43. Albright TS, Colombo DF, Euscher E, Samuels P. Evaluation of the effect of meconium on assessment of fetal lung maturity status by TDxFLM II testing. Obstet Gynecol 2004; 104:952.
  44. Wong SS, Schenkel O, Qutishat A. Strategic utilization of fetal lung maturity tests. Scand J Clin Lab Invest 1996; 56:525.
  45. Srouji SS, Carr DB, Gardella CM, et al. The effect of common clinical contaminants on amniotic fluid fluorescence polarization results. Obstet Gynecol 2004; 104:1237.
  46. Grenache DG, Parvin CA, Gronowski AM. Preanalytical factors that influence the Abbott TDx Fetal Lung Maturity II assay. Clin Chem 2003; 49:935.
  47. Spillman T, Cotton DB. Current perspectives in assessment of fetal pulmonary surfactant status with amniotic fluid. Crit Rev Clin Lab Sci 1989; 27:341.
  48. Jobe AH. Fetal lung development, tests for maturation, induction of maturation and treatment. In: Maternal-Fetal Medicine, Creasy RK, Resnik R (Eds), WB Saunders, Philadelphia 1999. p.417.
  49. Piazze JJ, Maranghi L, Cosmi EV, Anceschi MM. The effect of polyhydramnios and oligohydramnios on fetal lung maturity indexes. Am J Perinatol 1998; 15:249.
  50. Wijnberger LD, de Kleine M, Voorbij HA, et al. The effect of clinical characteristics on the lecithin/sphingomyelin ratio and lamellar body count: a cross-sectional study. J Matern Fetal Neonatal Med 2003; 14:373.
  51. Edwards RK, Duff P, Ross KC. Amniotic fluid indices of fetal pulmonary maturity with preterm premature rupture of membranes. Obstet Gynecol 2000; 96:102.
  52. Cleary-Goldman J, Connolly T, Chelmow D, Malone F. Accuracy of the TDx-FLM assay of amniotic fluid: a comparison of vaginal pool samples with amniocentesis. J Matern Fetal Neonatal Med 2002; 11:374.
  53. Dombroski RA, MacKenna J, Brame RG. Comparison of amniotic fluid lung maturity profiles in paired vaginal and amniocentesis specimens. Am J Obstet Gynecol 1981; 140:461.
  54. Lambers DS, Brady K, Leist PA, et al. Ability of normal vaginal flora to produce detectable phosphatidylglycerol in amniotic fluid in vitro. Obstet Gynecol 1995; 85:651.
  55. Schumacher RE, Parisi VM, Steady HM, Tsao FH. Bacteria causing false positive test for phosphatidylglycerol in amniotic fluid. Am J Obstet Gynecol 1985; 151:1067.
  56. Tanasijevic MJ, Winkelman JW, Wybenga DR, et al. Prediction of fetal lung maturity in infants of diabetic mothers using the FLM S/A and disaturated phosphatidylcholine tests. Am J Clin Pathol 1996; 105:17.
  57. Livingston EG, Herbert WN, Hage ML, et al. Use of the TDx-FLM assay in evaluating fetal lung maturity in an insulin-dependent diabetic population. The Diabetes and Fetal Maturity Study Group. Obstet Gynecol 1995; 86:826.
  58. Curet LB, Tsao FH, Zachman RD, et al. Phosphatidylglycerol, lecithin/sphingomyelin ratio and respiratory distress syndrome in diabetic and non-diabetic pregnancies. Int J Gynaecol Obstet 1989; 30:105.
  59. Berkowitz K, Reyes C, Saadat P, Kjos SL. Fetal lung maturation. Comparison of biochemical indices in gestational diabetic and nondiabetic pregnancies. J Reprod Med 1997; 42:793.
  60. Melanson SE, Jarolim P, McElrath TF. Fetal lung maturity testing in diabetic mothers. Lab Med 2007; 38:553.